No hay diferencias pre 6 y 24 hrs - tài liệu tham khảo | Đại học Hoa Sen

No hay diferencias pre 6 y 24 hrs - tài liệu tham khảo | Đại học Hoa Sen và thông tin bổ ích giúp sinh viên tham khảo, ôn luyện và phục vụ nhu cầu học tập của mình cụ thể là có định hướng, ôn tập, nắm vững kiến thức môn học và làm bài tốt trong những bài kiểm tra, bài tiểu luận, bài tập kết thúc học phần, từ đó học tập tốt và có kết quả cao cũng như có thể vận dụng tốt những kiến thức

Article ID: WMC001717 ISSN 2046-1690
Characterization of the Effects of One Maximal
Repetition Test on Muscle Injury and Inflammation
Markers
Corresponding Author:
Dr. Sandro M Hirabara,
Professor, ICAFE, Cruzeiro do Sul University, Rua Galvão Bueno, 868, 01506-000 - Brazil
Submitting Author:
Dr. Sandro M Hirabara,
Professor, ICAFE, Cruzeiro do Sul University, Rua Galvão Bueno, 868, 01506-000 - Brazil
Article ID: WMC001717
Article Type: Research articles
Submitted on: Published on:09-Mar-2011, 07:04:41 PM GMT 10-Mar-2011, 09:56:03 PM GMT
Article URL: http://www.webmedcentral.com/article_view/1717
Subject Categories:PHYSIOLOGY
Keywords:One Repetition Maximum Test, Muscle Damage, Inflammation, Creatine Kinase, C-Reactive Protein,
How to cite the article:Barquilha G , Uchida M C, Santos V C, Moura N R, Lambertucci R H, Hatanaka E ,
Cury-Boaventura M F, Pithon-Curi T C, Gorjão R , Hirabara S M. Characterization of the Effects of One Maximal
Repetition Test on Muscle Injury and Inflammation Markers . WebmedCentral PHYSIOLOGY
2011;2(3):WMC001717
Source(s) of Funding:
This work was supported by CNPq, FAPESP and CAPES.
Competing Interests:
This study has no competing interests.
WebmedCentral > Research articles Page 1 of 8
WMC001717 Downloaded from http://www.webmedcentral.com on 07-Sep-2011, 11:54:03 AM
Characterization of the Effects of One Maximal
Repetition Test on Muscle Injury and Inflammation
Markers
Author(s): Barquilha G , Uchida M C, Santos V C, Moura N R, Lambertucci R H, Hatanaka E , Cury-Boaventura
M F, Pithon-Curi T C, Gorjão R , Hirabara S M
Abstract
We assessed the influence of the one repetition
maximum (1RM) bench press exercise on the
temporal profile of markers of muscular injury (creatine
kinase - CK - activity) and inflammation (interleukin-2 -
IL-2, IL-1 , tumoral necrose factor-a - TNF- , IL-6, and
C-reactive protein - CRP). Participants were 11
healthy subjects both genders (eight men and three
women), active, involved in strength training, for
recreational purposes for more than six months.
Characteristics of the volunteers were: age (21.5 ± 6.5
years), weight (70.1 ± 21.1 kg), height (172.8 ± 52.1
cm), and fat mass (14.5 ± 5.7 %). Sample blood was
colleted before the 1 RM test and at 1 h, 24 h, 48 h,
and 6 days after the test. Statistical analysis was
performed using ANOVA with repeated measurements
and Bonferroni post-test. There was a significant
increase in the CK activity after 6 days of the test
when compared to pre-test time, whereas CRP activity
increased 24 and 48 hours when compared to pre-test
period. There was not significant difference in the
plasma cytokine levels. Although 1 RM test did not
alter the levels of inflammatory cytokines, it can be
observed through this work that this test can induce
muscle damage, which would be a negative factor for
athletes, since the muscle injury and inflammation are
associated with decreased performance, especially
strength and muscle power.
Introduction
Before the prescription of resistance exercise, it is
common to use some tests in order to evaluate muscle
strength and track, as for example the one repetition
maximum test (1 RM test). This test, by raising the
maximum weight possible in a single complete
movement, aims to stimulate the dynamic maximum
strength by the practitioner (Ware et al. 1995). The 1
RM test has several advantages, such as low cost,
easy implementation, specificity, and ability to adapt to
reality of various sports (Barnard et al. 1999). However,
physiological changes induced by the 1 RM test have
been poorly studied. Here, we evaluated the effects of
1 RM on muscle injury and inflammatory markers in
healthy subjects.
Resistance exercise has been shown to be an
important intervention for promoting and maintaining
health and quality of life. However, the physiological
changes promoted by resistance exercise are under
investigation yet. It is noteworthy to date that no
studies are found about the muscle damage and
inflammation caused by the 1RM test in healthy
subjects.
Analysis of markers of m uscular i njury and
inflammation is frequently used to investigate whether
there is muscle damage induced by exercise (Glesson
2002; Margonis et al. 2007; Uchida et al. 2009). The
activity of the creatine kinase (CK) in blood has been
used as a main surrogate marker of muscle damage,
resulting from eccentric exercise (Stupka et al. 2000;
Totsuka et al. 2002; Evans et al. 2002; Nosaka et al.
2002a; Nosaka et al. 2002b; Tartibian et al. 2009). The
increase CK activity in plasma indicates that there was
a release of the enzyme due to a rupture of the muscle
cell membrane (muscle damage), considering that CK
does not have the ability to cross the membrane when
the sarcoplasm is intact (Brown et al. 1997;
Brancaccio et al. 2007). Increased plasma activity of
this enzyme is influenced by both the volume and
intensity of exercise (Tiidus and Ianuzzo 1983; Uchida
et al. 2009).
Tissue damage leads to activation of the immune
defense cells, the leukocytes, in order to remove
unwanted elements arising from such injury (Pyne
1994). When activated, the leukocytes can stimulate
the release of proinflammatory cytokines such as
tumor necrosis factor- (TNF- ) and interleukins (IL),
IL-8 and IL-6. These factors stimulate the release of
anti-inflammatory cytokines as IL-1ra and may also
stimulate the acute phase proteins, such as C-reactive
protein (CRP) (Nieman et al. 2005; Smith 2000b;
Robson 2003; Steensberg et al. 2003).
Abrupt increases in markers of muscle damage and
inflammation resulting from intense muscle effort can
affect the immune system and metabolism, impairing
the performance of athletes (Nieman 2007;
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Steensberg et al. 2003). The 1 RM test is greatly and
frequently used for measuring muscle strength, but its
effects on muscle damage and inflammatory process
is not known yet. Thus, the purpose of this study was
to assess the influence of the 1RM bench press
exercise on the temporal profile of markers of
muscular injury and inflammation in healthy subjects.
Methods
Subjects
Participants of the study were 11 healthy subjects both
genders (eight men and three women), active,
involved in strength training, for recreational purposes
for more than six months. Characteristics of the
volunteers were: age (21.5 ± 6.5 years), weight (70.1
± 21.1 kg), height (172.8 ± 52.1 cm), and fat mass
(14.5 ± 5.7 %). The subjects voluntarily signed a
consent form. This study was approved by the ethics
committee and research of the Cruzeiro do Sul
University (protocol No. 039/2009).
Exercise protocol (1 RM test)
For the determination of 1 RM in a bench press
exercise, participants were instructed to grip the bar at
a comfortable position, which was typically 10 to 20
cm wider than shoulder width (Kim et al. 2002).
Subjects performed a warm-up consisting in 3 sets: 1
st
set: 8–10 repetitions using a light weight (~ 50% of 1
RM); 2 set: 3–5 repetitions using a moderate weight
nd
(~ 70% 1 of RM); 3 set: 1–3 repetitions using a heavy
rd
weight (~ 80% of 1RM). After the warm-up, each
participant was submitted to the 1 RM test by
increasing the resistance on subsequent attempts until
he or she was unable to finalize a full correct
movement. Approximately 5 sets of one repetition
were accomplished and each attempt was separated
by 3 min of rest (Shimano et al. 2006). The 1 RM tests
were accomplished by two trained spotters.
Determination of the CK, plasma interleukin
concentrations and serum CRP
CK activity and CRP were determined by a highly
sensitive immunoturbidimetric method (Bioclin
Diagnostics, São Paulo, Brazil), according to the
manufacturer’s instructions. Plasma concentrations of
IL-6, IL-8, TNF- , and IL-1ra were determined based
on the enzyme-linked immunosorbent assay (ELISA),
using a Duoset Kit (Quantikine, R&D Systems,
Minneapolis, MM, USA), following the manufacturer’s
instructions.
Statistical analysis
Analysis of variance (ANOVA) with repeated
mesuarements, followed by the Tukey post-test, was
performed to verify the statistical differences. Criterion
for statistical significance was set at p <0.05.
Results
Illustration 1 shows the activity of CK and the
concentration of CRP in plasma (Illustration 1A and 1B,
respectively). The 1 RM test increased the CK activity
after 24 h, remaining elevated up to 6 days after the
test (p<0.05) in a time-dependent manner (Ilustration
1A). CRP concentration was elevated 24 h after the 1
RM test (p<0.001), remained elevated up to 48 h
(p<0.001) and returned to the basal value at 6 days
after the test (Illustration 1B). No changes in the
pro-inflammatory cytokines IL-6, TNF-a, IL-8 and
IL-1Ra induced by 1 RM test were found (Illustration
2).
Discussion
Although a growing number of studies focusing the
changes in markers of inflammation and injury during
and after resistance exercise, nothing is known about
the physiological aspect of the test after completion of
a 1 RM test, one exercise of high intensity and low
volume. In this study, we found a linear increase in
activity of CK at the times 24 h, 48 h, and 6 days after
the 1 RM test. Studies have shown that CK may
remain increased up to 7 days after the execution of
an effort (Brancaccio et al. 2007; Bruunsgaard et al.
1997).
Uchida et al. (2009) conducted a study which aimed to
investigate muscle damage in different intensities in
bench press exercise. The intensities were 50%, 75%,
90% and 110% of 1 RM. The activity of CK enzyme
increased significantly in all groups after bout, with no
significant difference among groups, probably because
the total volumes were similar among them. Already
Paschalis and colleagues (Paschalis et al. 2005)
compared two different protocols of resistance
exercise, with a moderate and one with high intensity,
finding a significant increase of CK in both protocols.
But, it is noteworthy that the highest value of CK
activity presented in this study was found in the group
who performed intense exercise, showing that the
intensity of exercise is the major factor in modulating
the response of CK activity.
Were also evaluated some markers of muscle
inflammation. These markers were cytokines (IL-8,
IL1R-a, TNF- and IL-6), which did not show
significant changes with the test of 1RM. Our results
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corroborate the findings of Uchida et al. (2009) and
Hirose et al. (2004), who also found no significant
changes of the cytokines analyzed with a protocol of
strength training. The Ushida’study (2009) evaluated
the cytokines IL-6 and IL-1 , in addition to TNF- . No
significant change was found in none of these
cytokines in any of intensities studied (50, 75, 90 and
110% of 1 RM). Hirose et al. (2004) investigated the
effect of a protocol of eccentric exercise (6 sets of 5
repetitions in exercise of elbow flexors) in several
cytokines, among them the IL-1ra, IL-6, IL-8 and TNF-
. These cytokines, in corroborating with our results,
were not significantly modified by the eccentric
exercise. Changes in plasma cytokines have been
found in exercises cyclic bulk (Toft et al. 2000; Nieman
2001). Thus, one possible reason for the lack of
alteration of cytokines in our study is the small volume
used by the 1 RM test. In addition, an important factor
that should be taken into account is the difficulty in
detecting cytokines in plasma, due to the short time
that they are stable (Petersen and Pedersen 2005).
In the present study, we found an increase in acute
phase protein CRP, which peaked at 48 hours after te
1 RM test. CRP has proinflammatory characteristics
(activa t ion of the complement system and
opsonization of bacteria) and anti-inflammatory (to
prevent the adhesion of neutrophils to endothelial cells,
inhibit the generation of superoxide by neutrophils and
stimulating the synthesis of the receptor antagonist
IL-1) (du Closs 2000; Epstein 1999, Semple et al.
2004). Taylor et al. (1987) found an increase of 300%,
24 hours after performing a triathlon race. Semple and
colleagues (2004) also found a significant increase of
this protein after an ultramarathon. It is well known that
the cytokines IL-6 and TNF- stimulate the production
of acute-phase proteins, such as CRP (Semple et al.
2004; Du Closs 2000). In our study we found a
significant increase in CRP, but was not detected
increased IL-6 and TNF- , possibly because the CRP
is more stable in plasma than cytokines, which are
more difficult to detect (Pedersen 2005).
In conclusion, the 1 RM test (a session of high
intensity, but low volume) was enough to increase CK
activity and CRP concentration in the plasma. These
results suggest that indeed there were muscle
damage and inflammatory response (increased CRP)
after the 1 RM test. Concentration of pro-inflammatory
cytokines, however, was not modified by the test.
Conclusion(s)
The 1RM test is widely used by athletes of various
sports. It can be observed through this work that this
test of high intensity and low volume can lead to
induction of muscle damage, which would be a
negative factor for the athletes, since the muscle injury
and inflammation are associated with decreased
performance, especially strength and muscle power.
Therefore, special care must be taken in introducing
the 1RM test in sports periodization.
Abbreviation(s)
1 RM, one repetition maximum; IL, interleucin; TNF,
tumoral necrosis factor; CK, creatine kinase; CRP,
C-reactive protein
Acknowledgement(s)
This study was supported by grants from FAPESP,
CNPq, and CAPES.
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Illustrations
Illustration 1
??Means and standard deviations of the activity of CK (A) and concentration of CRP (B) at
baseline (pre) and after the 1RM test session at 1 h (post), 24 h, 48 h, and 6 days.
Illustration 2
Mean (and range) of the concentrations of L-6, IL-8, IL-1ra and TNF-a at baseline (pre) and
after the 1 RM test session at 1 h, (post), 24 h, and 48 h.
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Article ID: WMC001717 ISSN 2046-1690
Characterization of the Effects of One Maximal
Repetition Test on Muscle Injury and Inflammation Markers
Corresponding Author: Dr. Sandro M Hirabara,
Professor, ICAFE, Cruzeiro do Sul University, Rua Galvão Bueno, 868, 01506-000 - Brazil Submitting Author: Dr. Sandro M Hirabara,
Professor, ICAFE, Cruzeiro do Sul University, Rua Galvão Bueno, 868, 01506-000 - Brazil Article ID: WMC001717
Article Type: Research articles
Submitted on:09-Mar-2011, 07:04:41 PM GMT Published on: 10-Mar-2011, 09:56:03 PM GMT
Article URL: http://www.webmedcentral.com/article_view/1717
Subject Categories:PHYSIOLOGY
Keywords:One Repetition Maximum Test, Muscle Damage, Inflammation, Creatine Kinase, C-Reactive Protein,
How to cite the article:Barquilha G , Uchida M C, Santos V C, Moura N R, Lambertucci R H, Hatanaka E ,
Cury-Boaventura M F, Pithon-Curi T C, Gorjão R , Hirabara S M. Characterization of the Effects of One Maximal
Repetition Test on Muscle Injury and Inflammation Markers . WebmedCentral PHYSIOLOGY 2011;2(3):WMC001717 Source(s) of Funding:
This work was supported by CNPq, FAPESP and CAPES. Competing Interests:
This study has no competing interests.
WebmedCentral > Research articles Page 1 of 8 WMC001717
Downloaded from http://www.webmedcentral.com on 07-Sep-2011, 11:54:03 AM
Characterization of the Effects of One Maximal
Repetition Test on Muscle Injury and Inflammation Markers

Author(s): Barquilha G , Uchida M C, Santos V C, Moura N R, Lambertucci R H, Hatanaka E , Cury-Boaventura
M F, Pithon-Curi T C, Gorjão R , Hirabara S M Abstract
been poorly studied. Here, we evaluated the effects of
1 RM on muscle injury and inflammatory markers in healthy subjects.
We assessed the influence of the one repetition
Resistance exercise has been shown to be an
maximum (1RM) bench press exercise on the
important intervention for promoting and maintaining
temporal profile of markers of muscular injury (creatine
health and quality of life. However, the physiological
kinase - CK - activity) and inflammation (interleukin-2 -
changes promoted by resistance exercise are under
IL-2, IL-1 , tumoral necrose factor-a - TNF- , IL-6, and
investigation yet. It is noteworthy to date that no
C-reactive protein - CRP). Participants were 11
studies are found about the muscle damage and
healthy subjects both genders (eight men and three
inflammation caused by the 1RM test in healthy
women), active, involved in strength training, for subjects.
recreational purposes for more than six months.
Analysis of markers of muscular injury and
Characteristics of the volunteers were: age (21.5 ± 6.5
inflammation is frequently used to investigate whether
years), weight (70.1 ± 21.1 kg), height (172.8 ± 52.1
there is muscle damage induced by exercise (Glesson
cm), and fat mass (14.5 ± 5.7 %). Sample blood was
2002; Margonis et al. 2007; Uchida et al. 2009). The
colleted before the 1 RM test and at 1 h, 24 h, 48 h,
activity of the creatine kinase (CK) in blood has been
and 6 days after the test. Statistical analysis was
used as a main surrogate marker of muscle damage,
performed using ANOVA with repeated measurements
resulting from eccentric exercise (Stupka et al. 2000;
and Bonferroni post-test. There was a significant
Totsuka et al. 2002; Evans et al. 2002; Nosaka et al.
increase in the CK activity after 6 days of the test
2002a; Nosaka et al. 2002b; Tartibian et al. 2009). The
when compared to pre-test time, whereas CRP activity
increase CK activity in plasma indicates that there was
increased 24 and 48 hours when compared to pre-test
a release of the enzyme due to a rupture of the muscle
period. There was not significant difference in the
cell membrane (muscle damage), considering that CK
plasma cytokine levels. Although 1 RM test did not
does not have the ability to cross the membrane when
alter the levels of inflammatory cytokines, it can be
the sarcoplasm is intact (Brown et al. 1997;
observed through this work that this test can induce
Brancaccio et al. 2007). Increased plasma activity of
muscle damage, which would be a negative factor for
this enzyme is influenced by both the volume and
athletes, since the muscle injury and inflammation are
intensity of exercise (Tiidus and Ianuzzo 1983; Uchida
associated with decreased performance, especially et al. 2009). strength and muscle power.
Tissue damage leads to activation of the immune Introduction
defense cells, the leukocytes, in order to remove
unwanted elements arising from such injury (Pyne
1994). When activated, the leukocytes can stimulate
the release of proinflammatory cytokines such as
Before the prescription of resistance exercise, it is
tumor necrosis factor- (TNF- ) and interleukins (IL),
common to use some tests in order to evaluate muscle
IL-8 and IL-6. These factors stimulate the release of
strength and track, as for example the one repetition
anti-inflammatory cytokines as IL-1ra and may also
maximum test (1 RM test). This test, by raising the
stimulate the acute phase proteins, such as C-reactive
maximum weight possible in a single complete
protein (CRP) (Nieman et al. 2005; Smith 2000b;
movement, aims to stimulate the dynamic maximum
Robson 2003; Steensberg et al. 2003).
strength by the practitioner (Ware et al. 1995). The 1
RM test has several advantages, such as low cost,
Abrupt increases in markers of muscle damage and
easy implementation, specificity, and ability to adapt to
inflammation resulting from intense muscle effort can
reality of various sports (Barnard et al. 1999). However,
affect the immune system and metabolism, impairing
physiological changes induced by the 1 RM test have
the performance of athletes (Nieman 2007;
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Steensberg et al. 2003). The 1 RM test is greatly and
mesuarements, followed by the Tukey post-test, was
frequently used for measuring muscle strength, but its
performed to verify the statistical differences. Criterion
effects on muscle damage and inflammatory process
for statistical significance was set at p <0.05.
is not known yet. Thus, the purpose of this study was Results
to assess the influence of the 1RM bench press
exercise on the temporal profile of markers of
muscular injury and inflammation in healthy subjects.
Illustration 1 shows the activity of CK and the Methods
concentration of CRP in plasma (Illustration 1A and 1B,
respectively). The 1 RM test increased the CK activity
after 24 h, remaining elevated up to 6 days after the Subjects
test (p<0.05) in a time-dependent manner (Ilustration
Participants of the study were 11 healthy subjects both
1A). CRP concentration was elevated 24 h after the 1
genders (eight men and three women), active,
RM test (p<0.001), remained elevated up to 48 h
involved in strength training, for recreational purposes
(p<0.001) and returned to the basal value at 6 days
for more than six months. Characteristics of the
after the test (Illustration 1B). No changes in the
volunteers were: age (21.5 ± 6.5 years), weight (70.1
pro-inflammatory cytokines IL-6, TNF-a, IL-8 and
± 21.1 kg), height (172.8 ± 52.1 cm), and fat mass
IL-1Ra induced by 1 RM test were found (Illustration
(14.5 ± 5.7 %). The subjects voluntarily signed a 2).
consent form. This study was approved by the ethics Discussion
committee and research of the Cruzeiro do Sul
University (protocol No. 039/2009).
Exercise protocol (1 RM test)
Although a growing number of studies focusing the
For the determination of 1 RM in a bench press
changes in markers of inflammation and injury during
exercise, participants were instructed to grip the bar at
and after resistance exercise, nothing is known about
a comfortable position, which was typically 10 to 20
the physiological aspect of the test after completion of
cm wider than shoulder width (Kim et al. 2002).
a 1 RM test, one exercise of high intensity and low
Subjects performed a warm-up consisting in 3 sets: 1st
volume. In this study, we found a linear increase in
set: 8–10 repetitions using a light weight (~ 50% of 1
activity of CK at the times 24 h, 48 h, and 6 days after
RM); 2nd set: 3–5 repetitions using a moderate weight
the 1 RM test. Studies have shown that CK may
(~ 70% 1 of RM); 3rd set: 1–3 repetitions using a heavy
remain increased up to 7 days after the execution of
weight (~ 80% of 1RM). After the warm-up, each
an effort (Brancaccio et al. 2007; Bruunsgaard et al.
participant was submitted to the 1 RM test by 1997).
increasing the resistance on subsequent attempts until
Uchida et al. (2009) conducted a study which aimed to
he or she was unable to finalize a full correct
investigate muscle damage in different intensities in
movement. Approximately 5 sets of one repetition
bench press exercise. The intensities were 50%, 75%,
were accomplished and each attempt was separated
90% and 110% of 1 RM. The activity of CK enzyme
by 3 min of rest (Shimano et al. 2006). The 1 RM tests
increased significantly in all groups after bout, with no
were accomplished by two trained spotters.
significant difference among groups, probably because
Determination of the CK, plasma interleukin
the total volumes were similar among them. Already
concentrations and serum CRP
Paschalis and colleagues (Paschalis et al. 2005)
compared two different protocols of resistance
CK activity and CRP were determined by a highly
exercise, with a moderate and one with high intensity,
sensitive immunoturbidimetric method (Bioclin
finding a significant increase of CK in both protocols.
Diagnostics, São Paulo, Brazil), according to the
But, it is noteworthy that the highest value of CK
manufacturer’s instructions. Plasma concentrations of
IL-6, IL-8, TNF- , and IL-1ra were determined based
activity presented in this study was found in the group
who performed intense exercise, showing that the
on the enzyme-linked immunosorbent assay (ELISA),
intensity of exercise is the major factor in modulating
using a Duoset Kit (Quantikine, R&D Systems, the response of CK activity.
Minneapolis, MM, USA), following the manufacturer’s instructions.
Were also evaluated some markers of muscle
inflammation. These markers were cytokines (IL-8, Statistical analysis
IL1R-a, TNF- and IL-6), which did not show
Analysis of variance (ANOVA) with repeated
significant changes with the test of 1RM. Our results
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corroborate the findings of Uchida et al. (2009) and
test of high intensity and low volume can lead to
Hirose et al. (2004), who also found no significant
induction of muscle damage, which would be a
changes of the cytokines analyzed with a protocol of
negative factor for the athletes, since the muscle injury
strength training. The Ushida’study (2009) evaluated
and inflammation are associated with decreased
the cytokines IL-6 and IL-1 , in addition to TNF- . No
performance, especially strength and muscle power.
significant change was found in none of these
Therefore, special care must be taken in introducing
cytokines in any of intensities studied (50, 75, 90 and
the 1RM test in sports periodization.
110% of 1 RM). Hirose et al. (2004) investigated the Abbreviation(s)
effect of a protocol of eccentric exercise (6 sets of 5
repetitions in exercise of elbow flexors) in several
cytokines, among them the IL-1ra, IL-6, IL-8 and TNF-
. These cytokines, in corroborating with our results,
1 RM, one repetition maximum; IL, interleucin; TNF,
were not significantly modified by the eccentric
tumoral necrosis factor; CK, creatine kinase; CRP,
exercise. Changes in plasma cytokines have been C-reactive protein
found in exercises cyclic bulk (Toft et al. 2000; Nieman Acknowledgement(s)
2001). Thus, one possible reason for the lack of
alteration of cytokines in our study is the small volume
used by the 1 RM test. In addition, an important factor
that should be taken into account is the difficulty in
This study was supported by grants from FAPESP,
detecting cytokines in plasma, due to the short time CNPq, and CAPES.
that they are stable (Petersen and Pedersen 2005). References
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phase protein CRP, which peaked at 48 hours after te
1 RM test. CRP has proinflammatory characteristics
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Downloaded from http://www.webmedcentral.com on 07-Sep-2011, 11:54:03 AM Illustrations Illustration 1
??Means and standard deviations of the activity of CK (A) and concentration of CRP (B) at
baseline (pre) and after the 1RM test session at 1 h (post), 24 h, 48 h, and 6 days. Illustration 2
Mean (and range) of the concentrations of L-6, IL-8, IL-1ra and TNF-a at baseline (pre) and
after the 1 RM test session at 1 h, (post), 24 h, and 48 h.
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