lOMoAR cPSD| 47708777 Case study
Respomix AERO - assessment of the properties of inhibiting the growth of bacteria isolated from poultry 1. Introduction
Respomix AERO has a unique composition based on a mixture of essential oils
such as eucalyptus, thyme peppermint and high-quality plant extracts. These
components include compounds that have the following effects: anti-
inflammatory, immunostimulant, secretolytic, extending airways and
smooth muscle relaxants (Handbook of Essential Oils Science, Technology and
Application, Farhadi et al. 2017). These properties significantly help to clean
the airway by removing inflamma- tory secretions which facilitates breathing. 2. Aim The
aim of the study was to evaluate Respomix AERO formulation in terms of its
properties of inhibiting the growth of bacteria isolated from clinical cases of
inflammation of the respiratory system in various species of poultry. 3. Material and methods
Bacterial strains: 23 bacterial strains were isolated on selective microbiological media from poultry
with clinical cases indicating inflammation of respiratory tract of birds. The tested strains included
10 isolates from broiler turkey, 9 from laying hen, 3 from broiler breeder for meat pro- duction
and 1 from chicken broiler. Species of isolated strains were confirmed by a test using MALDI-TOF
MS technique (Bruker, Germany). Until the testing time, the strains were stored in a freezer, at -
80°C, on Viabank medium (OXOID, UK). Testing the biological activity of extracts: The test was
performed by determining the MIC - Minimal Inhibitory Concentration using the micro-dilution
method, in accordance with the reference document CLSI M07-A10 „Methods for Dilution
Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically; Approved Standard — Tenth
Edition, January 2015”, for the following bacterial species: Enterococcus cecorum, Enterococcus
hirae, Gallibacterium anatis, Staphylococcus aureus, Escherichia coli and Candida albicans. 4. Results 4% 9 % lOMoAR cPSD| 47708777
• tests provided 4 different values of MIC for
Respomix AERO i.e. 1 µl/ml, 2 µl/ml, 4µl/ml and 8µl/ml.
• 52% of the tested strains had the MIC value of 2 µl/ml.
• the lowest minimum inhibitory concentration of 1
µl/ml was obtained for strains of Pasteurella multocida. 1 µl/ml 8 µl/ml 2 µl/ml 4 µl/ml Graph 1. Summary of results lOMoAR cPSD| 47708777 B. 100 % bronchiseptica 75% 25 % ORT 50% 25% 25 % P. multocida 80% 20 % B. avium 100 % G. anatis E. cecorum 80% 20 % 1 µl/ml 2 µl/ml 4 µl/ml 8 µl/ml
Graph 2. MIC values obtained for individual bacterial species. 5. Conclusions
Analysing the above test results, it can be concluded that Respomix AERO indicate properties that
inhibit growth of potentially pathogenic bacteria, isolated from clinical cases of respiratory disease
symptoms in poultry. The preparation, in addition to its obvious secretolytic and mucolytic
properties, also inhibits the growth of bacteria. 6. Literature
• Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically;
Approved Standard Tenth Edition January 2015
• Handbook of Essential Oils Sience, Technology and Application K. Hüsnü et al. Taylor and Francis Group 2010)
• Essential oils in broiler nutrition Kyung-Woo Lee Department of Nutrition, Faculty of Veterinary
Medicine, Utrecht University, The Netherlands Utrecht 2002 Recommende for d trouble breathing www.pharmi u l l.e